Information:
There are two-time slots available for this workshop, as the same session will be delivered twice.
During the registration process, you should select only the ONE-time slot that works best for you.
Fluorescence Microscopy and applications to microbiology
Date: 24 July 2024
Location: Faculdade de Ciências, Universidade de Lisboa, Portugal
C8 building, Room 8.2.47
Capacity: 20 attendees per session (2 sessions)
Description:
The objective of this workshop is to provide participants with fundamental knowledge on fluorescence microscopy for microbiology experiments. Each session will begin by exploring some of the most important properties of light and fluorescence in particular. Then, the instrumentation required for epifluorescence and confocal microscopy will be presented and explained. The participants will discuss which considerations need to be taken when designing, performing or analyzing microscopy experiments with bacterial or fungal samples. The session will end with a tour through the imaging facility, where epifluorescence, confocal and high-throughput microscopes will be presented along with typical results for each system.
Topics:
- Fundamental properties of light
- Main families of fluorophores
- Introduction to fluorescence microscopy
- Anatomy of the epifluorescence microscope
- Confocal microscopy
- Best practices for imaging microbiological samples
Tour of the imaging facility
Timetable
Instructor : Hugo Botelho – hmbotelho@ciencias.ulisboa.pt
Imaging Facility Manager | Junior Researcher
BioISI – Biosystems & Integrative Sciences Institute
Faculty of Sciences of the University of Lisboa, Lisboa/Portugal
Expertise: Microscopy, high-content screening, bioimage analysis, cystic fibrosis, drug discovery.
Personal Profile
Hugo M. Botelho is a Junior Researcher at the Biosystems & Integrative Sciences Institute (BioISI) of the Faculty of Sciences University of Lisbon (FCUL) and Manager of BioISI’s High-Throughput Screening (HTS) Facility. Scientist with a strong expertise in high-content screening, microscopy, cell biology, bioimage analysis and drug discovery.
His main research interest is the identification and characterization of novel therapeutic targets for the genetic disease Cystic Fibrosis (CF) through siRNA-based microscopy screens. He is also actively engaged in developing and deploying automated image analysis algorithms for high content microscopy, software development and data science approaches to biological data. At the HTS facility he is responsible for daily operations, instrument maintenance, user training and bioimage analysis support. His main microscopy expertise is on brightfield, epifluorescence, confocal, fluorescence lifetime imaging and high-throughput microscopy. He represents BioISI and FCUL at national and European research infrastructures: the Portuguese Platform of BioImaging (PPBI), PT-OPENSCREEN, Euro-BioImaging ERIC and EU-OPENSCREEN ERIC. He is a regular trainer and speaker at microscopy and CF meetings and courses.
