Information:

There are two-time slots available for this workshop, as the same session will be delivered twice.
During the registration process, you should select only the ONE-time slot that works best for you.

Fluorescence Microscopy and applications to microbiology

Date: 24 July 2024

Location: Faculdade de Ciências, Universidade de Lisboa, Portugal

C1 building, 3th Floor, Room 1.3.20

Capacity: 20 attendees per session (2 sessions)

Description:

The objective of this workshop is to provide participants with fundamental knowledge on fluorescence microscopy for microbiology experiments. Each session will begin by exploring some of the most important properties of light and fluorescence in particular. Then, the instrumentation required for epifluorescence and confocal microscopy will be presented and explained. The participants will discuss which considerations need to be taken when designing, performing or analyzing microscopy experiments with bacterial or fungal samples. The session will end with a tour through the imaging facility, where epifluorescence, confocal and high-throughput microscopes will be presented along with typical results for each system.

Topics:

  • Fundamental properties of light
  • Main families of fluorophores
  • Introduction to fluorescence microscopy
  • Anatomy of the epifluorescence microscope
  • Confocal microscopy
  • Best practices for imaging microbiological samples

Tour of the imaging facility


Location: Faculdade de Ciências, Universidade de Lisboa, Portugal – C1 building, 3th Floor, Room 1.3.20

Timetable

Instructor : Hugo Botelho – hmbotelho@ciencias.ulisboa.pt